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inference of crispr editing (ice) software tool  (Synthego Inc)

 
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    Structured Review

    Synthego Inc inference of crispr editing (ice) software tool
    Inference Of Crispr Editing (Ice) Software Tool, supplied by Synthego Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/inference+of+crispr+editing+(ice)+software+tool/inference+of+crispr+edits++ice+/us12356910-1050-17-22
    Average 90 stars, based on 1 article reviews
    inference of crispr editing (ice) software tool - by Bioz Stars, 2026-11
    90/100 stars

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    Related Articles

    DNA Sequencing:

    Article Title: Gene-edited basil plants resistant to downy mildew
    Article Snippet: The DNA sequencing chromatogram data for each TO and T1 sample was analyzed using the Inference of CRISPR Editing (ICE) software tool (Synthego Performance Analysis, ICE Analysis.

    Article Title: Oct4 is a gatekeeper of epithelial identity by regulating cytoskeletal organization in skin keratinocytes.
    Article Snippet: Genomic DNA from each OCT4 knockout clone or WT was extracted, amplified as above, and analyzed by sanger sequencing (Macrogen, Europe) followed by inference of CRISPR editing software (ICE) (Synthego, CA, USA) analysis to validate every clonal edit (Figure S1B).

    Article Title: CRISPR-Cas9 globin editing can induce megabase-scale copy-neutral losses of heterozygosity in hematopoietic cells
    Article Snippet: The Inference of CRISPR Editing (ICE) software ( https://ice.synthego.com ) was used to determine the percentage of indels among polyclonal populations for all protocols allowing gRNA efficiencies by ICE (Synthego).

    Article Title: PEG-Delivered CRISPR-Cas9 Ribonucleoproteins System for Gene-Editing Screening of Maize Protoplasts
    Article Snippet: CRISPR-Cas9 DNA changes were calculated based on the insertions and deletions (In/Dels) around the cleavage site (3 bp upstream of the PAM sequence) using the Inference of CRISPR Editing Software—ICE software v.2 (Synthego Corporation, Palo Alto, CA, USA).

    Article Title: FOXD1 regulates cell division in clear cell renal cell carcinoma
    Article Snippet: Genomic DNA was extracted, PCR-amplified, sequenced using Sanger sequencing, and analyzed for editing efficiency using Synthego Inference of CRISPR Edits (ICE) software ( ice.synthego.com ).

    Article Title: CRISPR-Editing of Sweet Basil ( Ocimum basilicum L.) Homoserine Kinase Gene for Improved Downy Mildew Disease Resistance
    Article Snippet: The DNA sequencing chromatogram data for each T 0 and T 1 sample was analyzed using the Inference of CRISPR Editing (ICE) software tool (Synthego Performance Analysis, ICE Analysis.

    Article Title: The Solanum tuberosum GBSSI gene: a target for assessing gene and base editing in tetraploid potato.
    Article Snippet: Chromatograms from Sanger sequencing were analysed using the Inference of CRISPR Editing (ICE) software (Synthego, USA), an open-source and free-to-use Webbased tool (https ://ice.synth ego.com) (Hsiau et al. 2018).

    CRISPR:

    Article Title: Gene-edited basil plants resistant to downy mildew
    Article Snippet: The DNA sequencing chromatogram data for each TO and T1 sample was analyzed using the Inference of CRISPR Editing (ICE) software tool (Synthego Performance Analysis, ICE Analysis.

    Article Title: Oct4 is a gatekeeper of epithelial identity by regulating cytoskeletal organization in skin keratinocytes.
    Article Snippet: Genomic DNA from each OCT4 knockout clone or WT was extracted, amplified as above, and analyzed by sanger sequencing (Macrogen, Europe) followed by inference of CRISPR editing software (ICE) (Synthego, CA, USA) analysis to validate every clonal edit (Figure S1B).

    Article Title: CRISPR-Cas9 globin editing can induce megabase-scale copy-neutral losses of heterozygosity in hematopoietic cells
    Article Snippet: The Inference of CRISPR Editing (ICE) software ( https://ice.synthego.com ) was used to determine the percentage of indels among polyclonal populations for all protocols allowing gRNA efficiencies by ICE (Synthego).

    Article Title: PEG-Delivered CRISPR-Cas9 Ribonucleoproteins System for Gene-Editing Screening of Maize Protoplasts
    Article Snippet: CRISPR-Cas9 DNA changes were calculated based on the insertions and deletions (In/Dels) around the cleavage site (3 bp upstream of the PAM sequence) using the Inference of CRISPR Editing Software—ICE software v.2 (Synthego Corporation, Palo Alto, CA, USA).

    Article Title: FOXD1 regulates cell division in clear cell renal cell carcinoma
    Article Snippet: Genomic DNA was extracted, PCR-amplified, sequenced using Sanger sequencing, and analyzed for editing efficiency using Synthego Inference of CRISPR Edits (ICE) software ( ice.synthego.com ).

    Article Title: CRISPR-Editing of Sweet Basil ( Ocimum basilicum L.) Homoserine Kinase Gene for Improved Downy Mildew Disease Resistance
    Article Snippet: The DNA sequencing chromatogram data for each T 0 and T 1 sample was analyzed using the Inference of CRISPR Editing (ICE) software tool (Synthego Performance Analysis, ICE Analysis.

    Article Title: The Solanum tuberosum GBSSI gene: a target for assessing gene and base editing in tetraploid potato.
    Article Snippet: Chromatograms from Sanger sequencing were analysed using the Inference of CRISPR Editing (ICE) software (Synthego, USA), an open-source and free-to-use Webbased tool (https ://ice.synth ego.com) (Hsiau et al. 2018).

    Software:

    Article Title: Gene-edited basil plants resistant to downy mildew
    Article Snippet: The DNA sequencing chromatogram data for each TO and T1 sample was analyzed using the Inference of CRISPR Editing (ICE) software tool (Synthego Performance Analysis, ICE Analysis.

    Article Title: Oct4 is a gatekeeper of epithelial identity by regulating cytoskeletal organization in skin keratinocytes.
    Article Snippet: Genomic DNA from each OCT4 knockout clone or WT was extracted, amplified as above, and analyzed by sanger sequencing (Macrogen, Europe) followed by inference of CRISPR editing software (ICE) (Synthego, CA, USA) analysis to validate every clonal edit (Figure S1B).

    Article Title: CRISPR-Cas9 globin editing can induce megabase-scale copy-neutral losses of heterozygosity in hematopoietic cells
    Article Snippet: The Inference of CRISPR Editing (ICE) software ( https://ice.synthego.com ) was used to determine the percentage of indels among polyclonal populations for all protocols allowing gRNA efficiencies by ICE (Synthego).

    Article Title: PEG-Delivered CRISPR-Cas9 Ribonucleoproteins System for Gene-Editing Screening of Maize Protoplasts
    Article Snippet: CRISPR-Cas9 DNA changes were calculated based on the insertions and deletions (In/Dels) around the cleavage site (3 bp upstream of the PAM sequence) using the Inference of CRISPR Editing Software—ICE software v.2 (Synthego Corporation, Palo Alto, CA, USA).

    Article Title: FOXD1 regulates cell division in clear cell renal cell carcinoma
    Article Snippet: Genomic DNA was extracted, PCR-amplified, sequenced using Sanger sequencing, and analyzed for editing efficiency using Synthego Inference of CRISPR Edits (ICE) software ( ice.synthego.com ).

    Article Title: CRISPR-Editing of Sweet Basil ( Ocimum basilicum L.) Homoserine Kinase Gene for Improved Downy Mildew Disease Resistance
    Article Snippet: The DNA sequencing chromatogram data for each T 0 and T 1 sample was analyzed using the Inference of CRISPR Editing (ICE) software tool (Synthego Performance Analysis, ICE Analysis.

    Article Title: The Solanum tuberosum GBSSI gene: a target for assessing gene and base editing in tetraploid potato.
    Article Snippet: Chromatograms from Sanger sequencing were analysed using the Inference of CRISPR Editing (ICE) software (Synthego, USA), an open-source and free-to-use Webbased tool (https ://ice.synth ego.com) (Hsiau et al. 2018).

    Knock-Out:

    Article Title: Gene-edited basil plants resistant to downy mildew
    Article Snippet: The DNA sequencing chromatogram data for each TO and T1 sample was analyzed using the Inference of CRISPR Editing (ICE) software tool (Synthego Performance Analysis, ICE Analysis.

    Article Title: Oct4 is a gatekeeper of epithelial identity by regulating cytoskeletal organization in skin keratinocytes.
    Article Snippet: Genomic DNA from each OCT4 knockout clone or WT was extracted, amplified as above, and analyzed by sanger sequencing (Macrogen, Europe) followed by inference of CRISPR editing software (ICE) (Synthego, CA, USA) analysis to validate every clonal edit (Figure S1B).

    Article Title: CRISPR-Cas9 globin editing can induce megabase-scale copy-neutral losses of heterozygosity in hematopoietic cells
    Article Snippet: The Inference of CRISPR Editing (ICE) software ( https://ice.synthego.com ) was used to determine the percentage of indels among polyclonal populations for all protocols allowing gRNA efficiencies by ICE (Synthego).

    Article Title: PEG-Delivered CRISPR-Cas9 Ribonucleoproteins System for Gene-Editing Screening of Maize Protoplasts
    Article Snippet: CRISPR-Cas9 DNA changes were calculated based on the insertions and deletions (In/Dels) around the cleavage site (3 bp upstream of the PAM sequence) using the Inference of CRISPR Editing Software—ICE software v.2 (Synthego Corporation, Palo Alto, CA, USA).

    Article Title: FOXD1 regulates cell division in clear cell renal cell carcinoma
    Article Snippet: Genomic DNA was extracted, PCR-amplified, sequenced using Sanger sequencing, and analyzed for editing efficiency using Synthego Inference of CRISPR Edits (ICE) software ( ice.synthego.com ).

    Article Title: CRISPR-Editing of Sweet Basil ( Ocimum basilicum L.) Homoserine Kinase Gene for Improved Downy Mildew Disease Resistance
    Article Snippet: The DNA sequencing chromatogram data for each T 0 and T 1 sample was analyzed using the Inference of CRISPR Editing (ICE) software tool (Synthego Performance Analysis, ICE Analysis.

    Article Title: The Solanum tuberosum GBSSI gene: a target for assessing gene and base editing in tetraploid potato.
    Article Snippet: Chromatograms from Sanger sequencing were analysed using the Inference of CRISPR Editing (ICE) software (Synthego, USA), an open-source and free-to-use Webbased tool (https ://ice.synth ego.com) (Hsiau et al. 2018).

    Amplification:

    Article Title: Gene-edited basil plants resistant to downy mildew
    Article Snippet: The DNA sequencing chromatogram data for each TO and T1 sample was analyzed using the Inference of CRISPR Editing (ICE) software tool (Synthego Performance Analysis, ICE Analysis.

    Article Title: Oct4 is a gatekeeper of epithelial identity by regulating cytoskeletal organization in skin keratinocytes.
    Article Snippet: Genomic DNA from each OCT4 knockout clone or WT was extracted, amplified as above, and analyzed by sanger sequencing (Macrogen, Europe) followed by inference of CRISPR editing software (ICE) (Synthego, CA, USA) analysis to validate every clonal edit (Figure S1B).

    Article Title: CRISPR-Cas9 globin editing can induce megabase-scale copy-neutral losses of heterozygosity in hematopoietic cells
    Article Snippet: The Inference of CRISPR Editing (ICE) software ( https://ice.synthego.com ) was used to determine the percentage of indels among polyclonal populations for all protocols allowing gRNA efficiencies by ICE (Synthego).

    Article Title: PEG-Delivered CRISPR-Cas9 Ribonucleoproteins System for Gene-Editing Screening of Maize Protoplasts
    Article Snippet: CRISPR-Cas9 DNA changes were calculated based on the insertions and deletions (In/Dels) around the cleavage site (3 bp upstream of the PAM sequence) using the Inference of CRISPR Editing Software—ICE software v.2 (Synthego Corporation, Palo Alto, CA, USA).

    Article Title: FOXD1 regulates cell division in clear cell renal cell carcinoma
    Article Snippet: Genomic DNA was extracted, PCR-amplified, sequenced using Sanger sequencing, and analyzed for editing efficiency using Synthego Inference of CRISPR Edits (ICE) software ( ice.synthego.com ).

    Article Title: CRISPR-Editing of Sweet Basil ( Ocimum basilicum L.) Homoserine Kinase Gene for Improved Downy Mildew Disease Resistance
    Article Snippet: The DNA sequencing chromatogram data for each T 0 and T 1 sample was analyzed using the Inference of CRISPR Editing (ICE) software tool (Synthego Performance Analysis, ICE Analysis.

    Article Title: The Solanum tuberosum GBSSI gene: a target for assessing gene and base editing in tetraploid potato.
    Article Snippet: Chromatograms from Sanger sequencing were analysed using the Inference of CRISPR Editing (ICE) software (Synthego, USA), an open-source and free-to-use Webbased tool (https ://ice.synth ego.com) (Hsiau et al. 2018).

    Sequencing:

    Article Title: Gene-edited basil plants resistant to downy mildew
    Article Snippet: The DNA sequencing chromatogram data for each TO and T1 sample was analyzed using the Inference of CRISPR Editing (ICE) software tool (Synthego Performance Analysis, ICE Analysis.

    Article Title: Oct4 is a gatekeeper of epithelial identity by regulating cytoskeletal organization in skin keratinocytes.
    Article Snippet: Genomic DNA from each OCT4 knockout clone or WT was extracted, amplified as above, and analyzed by sanger sequencing (Macrogen, Europe) followed by inference of CRISPR editing software (ICE) (Synthego, CA, USA) analysis to validate every clonal edit (Figure S1B).

    Article Title: CRISPR-Cas9 globin editing can induce megabase-scale copy-neutral losses of heterozygosity in hematopoietic cells
    Article Snippet: The Inference of CRISPR Editing (ICE) software ( https://ice.synthego.com ) was used to determine the percentage of indels among polyclonal populations for all protocols allowing gRNA efficiencies by ICE (Synthego).

    Article Title: PEG-Delivered CRISPR-Cas9 Ribonucleoproteins System for Gene-Editing Screening of Maize Protoplasts
    Article Snippet: CRISPR-Cas9 DNA changes were calculated based on the insertions and deletions (In/Dels) around the cleavage site (3 bp upstream of the PAM sequence) using the Inference of CRISPR Editing Software—ICE software v.2 (Synthego Corporation, Palo Alto, CA, USA).

    Article Title: FOXD1 regulates cell division in clear cell renal cell carcinoma
    Article Snippet: Genomic DNA was extracted, PCR-amplified, sequenced using Sanger sequencing, and analyzed for editing efficiency using Synthego Inference of CRISPR Edits (ICE) software ( ice.synthego.com ).

    Article Title: CRISPR-Editing of Sweet Basil ( Ocimum basilicum L.) Homoserine Kinase Gene for Improved Downy Mildew Disease Resistance
    Article Snippet: The DNA sequencing chromatogram data for each T 0 and T 1 sample was analyzed using the Inference of CRISPR Editing (ICE) software tool (Synthego Performance Analysis, ICE Analysis.

    Article Title: The Solanum tuberosum GBSSI gene: a target for assessing gene and base editing in tetraploid potato.
    Article Snippet: Chromatograms from Sanger sequencing were analysed using the Inference of CRISPR Editing (ICE) software (Synthego, USA), an open-source and free-to-use Webbased tool (https ://ice.synth ego.com) (Hsiau et al. 2018).

    Control:

    Article Title: Gene-edited basil plants resistant to downy mildew
    Article Snippet: The DNA sequencing chromatogram data for each TO and T1 sample was analyzed using the Inference of CRISPR Editing (ICE) software tool (Synthego Performance Analysis, ICE Analysis.

    Article Title: Oct4 is a gatekeeper of epithelial identity by regulating cytoskeletal organization in skin keratinocytes.
    Article Snippet: Genomic DNA from each OCT4 knockout clone or WT was extracted, amplified as above, and analyzed by sanger sequencing (Macrogen, Europe) followed by inference of CRISPR editing software (ICE) (Synthego, CA, USA) analysis to validate every clonal edit (Figure S1B).

    Article Title: CRISPR-Cas9 globin editing can induce megabase-scale copy-neutral losses of heterozygosity in hematopoietic cells
    Article Snippet: The Inference of CRISPR Editing (ICE) software ( https://ice.synthego.com ) was used to determine the percentage of indels among polyclonal populations for all protocols allowing gRNA efficiencies by ICE (Synthego).

    Article Title: PEG-Delivered CRISPR-Cas9 Ribonucleoproteins System for Gene-Editing Screening of Maize Protoplasts
    Article Snippet: CRISPR-Cas9 DNA changes were calculated based on the insertions and deletions (In/Dels) around the cleavage site (3 bp upstream of the PAM sequence) using the Inference of CRISPR Editing Software—ICE software v.2 (Synthego Corporation, Palo Alto, CA, USA).

    Article Title: FOXD1 regulates cell division in clear cell renal cell carcinoma
    Article Snippet: Genomic DNA was extracted, PCR-amplified, sequenced using Sanger sequencing, and analyzed for editing efficiency using Synthego Inference of CRISPR Edits (ICE) software ( ice.synthego.com ).

    Article Title: CRISPR-Editing of Sweet Basil ( Ocimum basilicum L.) Homoserine Kinase Gene for Improved Downy Mildew Disease Resistance
    Article Snippet: The DNA sequencing chromatogram data for each T 0 and T 1 sample was analyzed using the Inference of CRISPR Editing (ICE) software tool (Synthego Performance Analysis, ICE Analysis.

    Article Title: The Solanum tuberosum GBSSI gene: a target for assessing gene and base editing in tetraploid potato.
    Article Snippet: Chromatograms from Sanger sequencing were analysed using the Inference of CRISPR Editing (ICE) software (Synthego, USA), an open-source and free-to-use Webbased tool (https ://ice.synth ego.com) (Hsiau et al. 2018).

    Clone Assay:

    Article Title: Gene-edited basil plants resistant to downy mildew
    Article Snippet: The DNA sequencing chromatogram data for each TO and T1 sample was analyzed using the Inference of CRISPR Editing (ICE) software tool (Synthego Performance Analysis, ICE Analysis.

    Article Title: Oct4 is a gatekeeper of epithelial identity by regulating cytoskeletal organization in skin keratinocytes.
    Article Snippet: Genomic DNA from each OCT4 knockout clone or WT was extracted, amplified as above, and analyzed by sanger sequencing (Macrogen, Europe) followed by inference of CRISPR editing software (ICE) (Synthego, CA, USA) analysis to validate every clonal edit (Figure S1B).

    Article Title: CRISPR-Cas9 globin editing can induce megabase-scale copy-neutral losses of heterozygosity in hematopoietic cells
    Article Snippet: The Inference of CRISPR Editing (ICE) software ( https://ice.synthego.com ) was used to determine the percentage of indels among polyclonal populations for all protocols allowing gRNA efficiencies by ICE (Synthego).

    Article Title: PEG-Delivered CRISPR-Cas9 Ribonucleoproteins System for Gene-Editing Screening of Maize Protoplasts
    Article Snippet: CRISPR-Cas9 DNA changes were calculated based on the insertions and deletions (In/Dels) around the cleavage site (3 bp upstream of the PAM sequence) using the Inference of CRISPR Editing Software—ICE software v.2 (Synthego Corporation, Palo Alto, CA, USA).

    Article Title: FOXD1 regulates cell division in clear cell renal cell carcinoma
    Article Snippet: Genomic DNA was extracted, PCR-amplified, sequenced using Sanger sequencing, and analyzed for editing efficiency using Synthego Inference of CRISPR Edits (ICE) software ( ice.synthego.com ).

    Article Title: CRISPR-Editing of Sweet Basil ( Ocimum basilicum L.) Homoserine Kinase Gene for Improved Downy Mildew Disease Resistance
    Article Snippet: The DNA sequencing chromatogram data for each T 0 and T 1 sample was analyzed using the Inference of CRISPR Editing (ICE) software tool (Synthego Performance Analysis, ICE Analysis.

    Article Title: The Solanum tuberosum GBSSI gene: a target for assessing gene and base editing in tetraploid potato.
    Article Snippet: Chromatograms from Sanger sequencing were analysed using the Inference of CRISPR Editing (ICE) software (Synthego, USA), an open-source and free-to-use Webbased tool (https ://ice.synth ego.com) (Hsiau et al. 2018).

    Methylation:

    Article Title: Gene-edited basil plants resistant to downy mildew
    Article Snippet: The DNA sequencing chromatogram data for each TO and T1 sample was analyzed using the Inference of CRISPR Editing (ICE) software tool (Synthego Performance Analysis, ICE Analysis.

    Article Title: Oct4 is a gatekeeper of epithelial identity by regulating cytoskeletal organization in skin keratinocytes.
    Article Snippet: Genomic DNA from each OCT4 knockout clone or WT was extracted, amplified as above, and analyzed by sanger sequencing (Macrogen, Europe) followed by inference of CRISPR editing software (ICE) (Synthego, CA, USA) analysis to validate every clonal edit (Figure S1B).

    Article Title: CRISPR-Cas9 globin editing can induce megabase-scale copy-neutral losses of heterozygosity in hematopoietic cells
    Article Snippet: The Inference of CRISPR Editing (ICE) software ( https://ice.synthego.com ) was used to determine the percentage of indels among polyclonal populations for all protocols allowing gRNA efficiencies by ICE (Synthego).

    Article Title: PEG-Delivered CRISPR-Cas9 Ribonucleoproteins System for Gene-Editing Screening of Maize Protoplasts
    Article Snippet: CRISPR-Cas9 DNA changes were calculated based on the insertions and deletions (In/Dels) around the cleavage site (3 bp upstream of the PAM sequence) using the Inference of CRISPR Editing Software—ICE software v.2 (Synthego Corporation, Palo Alto, CA, USA).

    Article Title: FOXD1 regulates cell division in clear cell renal cell carcinoma
    Article Snippet: Genomic DNA was extracted, PCR-amplified, sequenced using Sanger sequencing, and analyzed for editing efficiency using Synthego Inference of CRISPR Edits (ICE) software ( ice.synthego.com ).

    Article Title: CRISPR-Editing of Sweet Basil ( Ocimum basilicum L.) Homoserine Kinase Gene for Improved Downy Mildew Disease Resistance
    Article Snippet: The DNA sequencing chromatogram data for each T 0 and T 1 sample was analyzed using the Inference of CRISPR Editing (ICE) software tool (Synthego Performance Analysis, ICE Analysis.

    Article Title: The Solanum tuberosum GBSSI gene: a target for assessing gene and base editing in tetraploid potato.
    Article Snippet: Chromatograms from Sanger sequencing were analysed using the Inference of CRISPR Editing (ICE) software (Synthego, USA), an open-source and free-to-use Webbased tool (https ://ice.synth ego.com) (Hsiau et al. 2018).

    In Vitro:

    Article Title: Gene-edited basil plants resistant to downy mildew
    Article Snippet: The DNA sequencing chromatogram data for each TO and T1 sample was analyzed using the Inference of CRISPR Editing (ICE) software tool (Synthego Performance Analysis, ICE Analysis.

    Article Title: Oct4 is a gatekeeper of epithelial identity by regulating cytoskeletal organization in skin keratinocytes.
    Article Snippet: Genomic DNA from each OCT4 knockout clone or WT was extracted, amplified as above, and analyzed by sanger sequencing (Macrogen, Europe) followed by inference of CRISPR editing software (ICE) (Synthego, CA, USA) analysis to validate every clonal edit (Figure S1B).

    Article Title: CRISPR-Cas9 globin editing can induce megabase-scale copy-neutral losses of heterozygosity in hematopoietic cells
    Article Snippet: The Inference of CRISPR Editing (ICE) software ( https://ice.synthego.com ) was used to determine the percentage of indels among polyclonal populations for all protocols allowing gRNA efficiencies by ICE (Synthego).

    Article Title: PEG-Delivered CRISPR-Cas9 Ribonucleoproteins System for Gene-Editing Screening of Maize Protoplasts
    Article Snippet: CRISPR-Cas9 DNA changes were calculated based on the insertions and deletions (In/Dels) around the cleavage site (3 bp upstream of the PAM sequence) using the Inference of CRISPR Editing Software—ICE software v.2 (Synthego Corporation, Palo Alto, CA, USA).

    Article Title: FOXD1 regulates cell division in clear cell renal cell carcinoma
    Article Snippet: Genomic DNA was extracted, PCR-amplified, sequenced using Sanger sequencing, and analyzed for editing efficiency using Synthego Inference of CRISPR Edits (ICE) software ( ice.synthego.com ).

    Article Title: CRISPR-Editing of Sweet Basil ( Ocimum basilicum L.) Homoserine Kinase Gene for Improved Downy Mildew Disease Resistance
    Article Snippet: The DNA sequencing chromatogram data for each T 0 and T 1 sample was analyzed using the Inference of CRISPR Editing (ICE) software tool (Synthego Performance Analysis, ICE Analysis.

    Article Title: The Solanum tuberosum GBSSI gene: a target for assessing gene and base editing in tetraploid potato.
    Article Snippet: Chromatograms from Sanger sequencing were analysed using the Inference of CRISPR Editing (ICE) software (Synthego, USA), an open-source and free-to-use Webbased tool (https ://ice.synth ego.com) (Hsiau et al. 2018).

    Expressing:

    Article Title: Gene-edited basil plants resistant to downy mildew
    Article Snippet: The DNA sequencing chromatogram data for each TO and T1 sample was analyzed using the Inference of CRISPR Editing (ICE) software tool (Synthego Performance Analysis, ICE Analysis.

    Article Title: Oct4 is a gatekeeper of epithelial identity by regulating cytoskeletal organization in skin keratinocytes.
    Article Snippet: Genomic DNA from each OCT4 knockout clone or WT was extracted, amplified as above, and analyzed by sanger sequencing (Macrogen, Europe) followed by inference of CRISPR editing software (ICE) (Synthego, CA, USA) analysis to validate every clonal edit (Figure S1B).

    Article Title: CRISPR-Cas9 globin editing can induce megabase-scale copy-neutral losses of heterozygosity in hematopoietic cells
    Article Snippet: The Inference of CRISPR Editing (ICE) software ( https://ice.synthego.com ) was used to determine the percentage of indels among polyclonal populations for all protocols allowing gRNA efficiencies by ICE (Synthego).

    Article Title: PEG-Delivered CRISPR-Cas9 Ribonucleoproteins System for Gene-Editing Screening of Maize Protoplasts
    Article Snippet: CRISPR-Cas9 DNA changes were calculated based on the insertions and deletions (In/Dels) around the cleavage site (3 bp upstream of the PAM sequence) using the Inference of CRISPR Editing Software—ICE software v.2 (Synthego Corporation, Palo Alto, CA, USA).

    Article Title: FOXD1 regulates cell division in clear cell renal cell carcinoma
    Article Snippet: Genomic DNA was extracted, PCR-amplified, sequenced using Sanger sequencing, and analyzed for editing efficiency using Synthego Inference of CRISPR Edits (ICE) software ( ice.synthego.com ).

    Article Title: CRISPR-Editing of Sweet Basil ( Ocimum basilicum L.) Homoserine Kinase Gene for Improved Downy Mildew Disease Resistance
    Article Snippet: The DNA sequencing chromatogram data for each T 0 and T 1 sample was analyzed using the Inference of CRISPR Editing (ICE) software tool (Synthego Performance Analysis, ICE Analysis.

    Article Title: The Solanum tuberosum GBSSI gene: a target for assessing gene and base editing in tetraploid potato.
    Article Snippet: Chromatograms from Sanger sequencing were analysed using the Inference of CRISPR Editing (ICE) software (Synthego, USA), an open-source and free-to-use Webbased tool (https ://ice.synth ego.com) (Hsiau et al. 2018).

    Binding Assay:

    Article Title: Gene-edited basil plants resistant to downy mildew
    Article Snippet: The DNA sequencing chromatogram data for each TO and T1 sample was analyzed using the Inference of CRISPR Editing (ICE) software tool (Synthego Performance Analysis, ICE Analysis.

    Article Title: Oct4 is a gatekeeper of epithelial identity by regulating cytoskeletal organization in skin keratinocytes.
    Article Snippet: Genomic DNA from each OCT4 knockout clone or WT was extracted, amplified as above, and analyzed by sanger sequencing (Macrogen, Europe) followed by inference of CRISPR editing software (ICE) (Synthego, CA, USA) analysis to validate every clonal edit (Figure S1B).

    Article Title: CRISPR-Cas9 globin editing can induce megabase-scale copy-neutral losses of heterozygosity in hematopoietic cells
    Article Snippet: The Inference of CRISPR Editing (ICE) software ( https://ice.synthego.com ) was used to determine the percentage of indels among polyclonal populations for all protocols allowing gRNA efficiencies by ICE (Synthego).

    Article Title: PEG-Delivered CRISPR-Cas9 Ribonucleoproteins System for Gene-Editing Screening of Maize Protoplasts
    Article Snippet: CRISPR-Cas9 DNA changes were calculated based on the insertions and deletions (In/Dels) around the cleavage site (3 bp upstream of the PAM sequence) using the Inference of CRISPR Editing Software—ICE software v.2 (Synthego Corporation, Palo Alto, CA, USA).

    Article Title: FOXD1 regulates cell division in clear cell renal cell carcinoma
    Article Snippet: Genomic DNA was extracted, PCR-amplified, sequenced using Sanger sequencing, and analyzed for editing efficiency using Synthego Inference of CRISPR Edits (ICE) software ( ice.synthego.com ).

    Article Title: CRISPR-Editing of Sweet Basil ( Ocimum basilicum L.) Homoserine Kinase Gene for Improved Downy Mildew Disease Resistance
    Article Snippet: The DNA sequencing chromatogram data for each T 0 and T 1 sample was analyzed using the Inference of CRISPR Editing (ICE) software tool (Synthego Performance Analysis, ICE Analysis.

    Article Title: The Solanum tuberosum GBSSI gene: a target for assessing gene and base editing in tetraploid potato.
    Article Snippet: Chromatograms from Sanger sequencing were analysed using the Inference of CRISPR Editing (ICE) software (Synthego, USA), an open-source and free-to-use Webbased tool (https ://ice.synth ego.com) (Hsiau et al. 2018).

    Plasmid Preparation:

    Article Title: Gene-edited basil plants resistant to downy mildew
    Article Snippet: The DNA sequencing chromatogram data for each TO and T1 sample was analyzed using the Inference of CRISPR Editing (ICE) software tool (Synthego Performance Analysis, ICE Analysis.

    Article Title: Oct4 is a gatekeeper of epithelial identity by regulating cytoskeletal organization in skin keratinocytes.
    Article Snippet: Genomic DNA from each OCT4 knockout clone or WT was extracted, amplified as above, and analyzed by sanger sequencing (Macrogen, Europe) followed by inference of CRISPR editing software (ICE) (Synthego, CA, USA) analysis to validate every clonal edit (Figure S1B).

    Article Title: CRISPR-Cas9 globin editing can induce megabase-scale copy-neutral losses of heterozygosity in hematopoietic cells
    Article Snippet: The Inference of CRISPR Editing (ICE) software ( https://ice.synthego.com ) was used to determine the percentage of indels among polyclonal populations for all protocols allowing gRNA efficiencies by ICE (Synthego).

    Article Title: PEG-Delivered CRISPR-Cas9 Ribonucleoproteins System for Gene-Editing Screening of Maize Protoplasts
    Article Snippet: CRISPR-Cas9 DNA changes were calculated based on the insertions and deletions (In/Dels) around the cleavage site (3 bp upstream of the PAM sequence) using the Inference of CRISPR Editing Software—ICE software v.2 (Synthego Corporation, Palo Alto, CA, USA).

    Article Title: FOXD1 regulates cell division in clear cell renal cell carcinoma
    Article Snippet: Genomic DNA was extracted, PCR-amplified, sequenced using Sanger sequencing, and analyzed for editing efficiency using Synthego Inference of CRISPR Edits (ICE) software ( ice.synthego.com ).

    Article Title: CRISPR-Editing of Sweet Basil ( Ocimum basilicum L.) Homoserine Kinase Gene for Improved Downy Mildew Disease Resistance
    Article Snippet: The DNA sequencing chromatogram data for each T 0 and T 1 sample was analyzed using the Inference of CRISPR Editing (ICE) software tool (Synthego Performance Analysis, ICE Analysis.

    Article Title: The Solanum tuberosum GBSSI gene: a target for assessing gene and base editing in tetraploid potato.
    Article Snippet: Chromatograms from Sanger sequencing were analysed using the Inference of CRISPR Editing (ICE) software (Synthego, USA), an open-source and free-to-use Webbased tool (https ://ice.synth ego.com) (Hsiau et al. 2018).

    Mutagenesis:

    Article Title: Gene-edited basil plants resistant to downy mildew
    Article Snippet: The DNA sequencing chromatogram data for each TO and T1 sample was analyzed using the Inference of CRISPR Editing (ICE) software tool (Synthego Performance Analysis, ICE Analysis.

    Article Title: Oct4 is a gatekeeper of epithelial identity by regulating cytoskeletal organization in skin keratinocytes.
    Article Snippet: Genomic DNA from each OCT4 knockout clone or WT was extracted, amplified as above, and analyzed by sanger sequencing (Macrogen, Europe) followed by inference of CRISPR editing software (ICE) (Synthego, CA, USA) analysis to validate every clonal edit (Figure S1B).

    Article Title: CRISPR-Cas9 globin editing can induce megabase-scale copy-neutral losses of heterozygosity in hematopoietic cells
    Article Snippet: The Inference of CRISPR Editing (ICE) software ( https://ice.synthego.com ) was used to determine the percentage of indels among polyclonal populations for all protocols allowing gRNA efficiencies by ICE (Synthego).

    Article Title: PEG-Delivered CRISPR-Cas9 Ribonucleoproteins System for Gene-Editing Screening of Maize Protoplasts
    Article Snippet: CRISPR-Cas9 DNA changes were calculated based on the insertions and deletions (In/Dels) around the cleavage site (3 bp upstream of the PAM sequence) using the Inference of CRISPR Editing Software—ICE software v.2 (Synthego Corporation, Palo Alto, CA, USA).

    Article Title: FOXD1 regulates cell division in clear cell renal cell carcinoma
    Article Snippet: Genomic DNA was extracted, PCR-amplified, sequenced using Sanger sequencing, and analyzed for editing efficiency using Synthego Inference of CRISPR Edits (ICE) software ( ice.synthego.com ).

    Article Title: CRISPR-Editing of Sweet Basil ( Ocimum basilicum L.) Homoserine Kinase Gene for Improved Downy Mildew Disease Resistance
    Article Snippet: The DNA sequencing chromatogram data for each T 0 and T 1 sample was analyzed using the Inference of CRISPR Editing (ICE) software tool (Synthego Performance Analysis, ICE Analysis.

    Article Title: The Solanum tuberosum GBSSI gene: a target for assessing gene and base editing in tetraploid potato.
    Article Snippet: Chromatograms from Sanger sequencing were analysed using the Inference of CRISPR Editing (ICE) software (Synthego, USA), an open-source and free-to-use Webbased tool (https ://ice.synth ego.com) (Hsiau et al. 2018).

    Transformation Assay:

    Article Title: Gene-edited basil plants resistant to downy mildew
    Article Snippet: The DNA sequencing chromatogram data for each TO and T1 sample was analyzed using the Inference of CRISPR Editing (ICE) software tool (Synthego Performance Analysis, ICE Analysis.

    Article Title: Oct4 is a gatekeeper of epithelial identity by regulating cytoskeletal organization in skin keratinocytes.
    Article Snippet: Genomic DNA from each OCT4 knockout clone or WT was extracted, amplified as above, and analyzed by sanger sequencing (Macrogen, Europe) followed by inference of CRISPR editing software (ICE) (Synthego, CA, USA) analysis to validate every clonal edit (Figure S1B).

    Article Title: CRISPR-Cas9 globin editing can induce megabase-scale copy-neutral losses of heterozygosity in hematopoietic cells
    Article Snippet: The Inference of CRISPR Editing (ICE) software ( https://ice.synthego.com ) was used to determine the percentage of indels among polyclonal populations for all protocols allowing gRNA efficiencies by ICE (Synthego).

    Article Title: PEG-Delivered CRISPR-Cas9 Ribonucleoproteins System for Gene-Editing Screening of Maize Protoplasts
    Article Snippet: CRISPR-Cas9 DNA changes were calculated based on the insertions and deletions (In/Dels) around the cleavage site (3 bp upstream of the PAM sequence) using the Inference of CRISPR Editing Software—ICE software v.2 (Synthego Corporation, Palo Alto, CA, USA).

    Article Title: FOXD1 regulates cell division in clear cell renal cell carcinoma
    Article Snippet: Genomic DNA was extracted, PCR-amplified, sequenced using Sanger sequencing, and analyzed for editing efficiency using Synthego Inference of CRISPR Edits (ICE) software ( ice.synthego.com ).

    Article Title: CRISPR-Editing of Sweet Basil ( Ocimum basilicum L.) Homoserine Kinase Gene for Improved Downy Mildew Disease Resistance
    Article Snippet: The DNA sequencing chromatogram data for each T 0 and T 1 sample was analyzed using the Inference of CRISPR Editing (ICE) software tool (Synthego Performance Analysis, ICE Analysis.

    Article Title: The Solanum tuberosum GBSSI gene: a target for assessing gene and base editing in tetraploid potato.
    Article Snippet: Chromatograms from Sanger sequencing were analysed using the Inference of CRISPR Editing (ICE) software (Synthego, USA), an open-source and free-to-use Webbased tool (https ://ice.synth ego.com) (Hsiau et al. 2018).

    Polymerase Chain Reaction:

    Article Title: Gene-edited basil plants resistant to downy mildew
    Article Snippet: The DNA sequencing chromatogram data for each TO and T1 sample was analyzed using the Inference of CRISPR Editing (ICE) software tool (Synthego Performance Analysis, ICE Analysis.

    Article Title: Oct4 is a gatekeeper of epithelial identity by regulating cytoskeletal organization in skin keratinocytes.
    Article Snippet: Genomic DNA from each OCT4 knockout clone or WT was extracted, amplified as above, and analyzed by sanger sequencing (Macrogen, Europe) followed by inference of CRISPR editing software (ICE) (Synthego, CA, USA) analysis to validate every clonal edit (Figure S1B).

    Article Title: CRISPR-Cas9 globin editing can induce megabase-scale copy-neutral losses of heterozygosity in hematopoietic cells
    Article Snippet: The Inference of CRISPR Editing (ICE) software ( https://ice.synthego.com ) was used to determine the percentage of indels among polyclonal populations for all protocols allowing gRNA efficiencies by ICE (Synthego).

    Article Title: PEG-Delivered CRISPR-Cas9 Ribonucleoproteins System for Gene-Editing Screening of Maize Protoplasts
    Article Snippet: CRISPR-Cas9 DNA changes were calculated based on the insertions and deletions (In/Dels) around the cleavage site (3 bp upstream of the PAM sequence) using the Inference of CRISPR Editing Software—ICE software v.2 (Synthego Corporation, Palo Alto, CA, USA).

    Article Title: FOXD1 regulates cell division in clear cell renal cell carcinoma
    Article Snippet: Genomic DNA was extracted, PCR-amplified, sequenced using Sanger sequencing, and analyzed for editing efficiency using Synthego Inference of CRISPR Edits (ICE) software ( ice.synthego.com ).

    Article Title: CRISPR-Editing of Sweet Basil ( Ocimum basilicum L.) Homoserine Kinase Gene for Improved Downy Mildew Disease Resistance
    Article Snippet: The DNA sequencing chromatogram data for each T 0 and T 1 sample was analyzed using the Inference of CRISPR Editing (ICE) software tool (Synthego Performance Analysis, ICE Analysis.

    Article Title: The Solanum tuberosum GBSSI gene: a target for assessing gene and base editing in tetraploid potato.
    Article Snippet: Chromatograms from Sanger sequencing were analysed using the Inference of CRISPR Editing (ICE) software (Synthego, USA), an open-source and free-to-use Webbased tool (https ://ice.synth ego.com) (Hsiau et al. 2018).

    Microscopy:

    Article Title: Gene-edited basil plants resistant to downy mildew
    Article Snippet: The DNA sequencing chromatogram data for each TO and T1 sample was analyzed using the Inference of CRISPR Editing (ICE) software tool (Synthego Performance Analysis, ICE Analysis.

    Article Title: Oct4 is a gatekeeper of epithelial identity by regulating cytoskeletal organization in skin keratinocytes.
    Article Snippet: Genomic DNA from each OCT4 knockout clone or WT was extracted, amplified as above, and analyzed by sanger sequencing (Macrogen, Europe) followed by inference of CRISPR editing software (ICE) (Synthego, CA, USA) analysis to validate every clonal edit (Figure S1B).

    Article Title: CRISPR-Cas9 globin editing can induce megabase-scale copy-neutral losses of heterozygosity in hematopoietic cells
    Article Snippet: The Inference of CRISPR Editing (ICE) software ( https://ice.synthego.com ) was used to determine the percentage of indels among polyclonal populations for all protocols allowing gRNA efficiencies by ICE (Synthego).

    Article Title: PEG-Delivered CRISPR-Cas9 Ribonucleoproteins System for Gene-Editing Screening of Maize Protoplasts
    Article Snippet: CRISPR-Cas9 DNA changes were calculated based on the insertions and deletions (In/Dels) around the cleavage site (3 bp upstream of the PAM sequence) using the Inference of CRISPR Editing Software—ICE software v.2 (Synthego Corporation, Palo Alto, CA, USA).

    Article Title: FOXD1 regulates cell division in clear cell renal cell carcinoma
    Article Snippet: Genomic DNA was extracted, PCR-amplified, sequenced using Sanger sequencing, and analyzed for editing efficiency using Synthego Inference of CRISPR Edits (ICE) software ( ice.synthego.com ).

    Article Title: CRISPR-Editing of Sweet Basil ( Ocimum basilicum L.) Homoserine Kinase Gene for Improved Downy Mildew Disease Resistance
    Article Snippet: The DNA sequencing chromatogram data for each T 0 and T 1 sample was analyzed using the Inference of CRISPR Editing (ICE) software tool (Synthego Performance Analysis, ICE Analysis.

    Article Title: The Solanum tuberosum GBSSI gene: a target for assessing gene and base editing in tetraploid potato.
    Article Snippet: Chromatograms from Sanger sequencing were analysed using the Inference of CRISPR Editing (ICE) software (Synthego, USA), an open-source and free-to-use Webbased tool (https ://ice.synth ego.com) (Hsiau et al. 2018).

    Transfection:

    Article Title: Gene-edited basil plants resistant to downy mildew
    Article Snippet: The DNA sequencing chromatogram data for each TO and T1 sample was analyzed using the Inference of CRISPR Editing (ICE) software tool (Synthego Performance Analysis, ICE Analysis.

    Article Title: Oct4 is a gatekeeper of epithelial identity by regulating cytoskeletal organization in skin keratinocytes.
    Article Snippet: Genomic DNA from each OCT4 knockout clone or WT was extracted, amplified as above, and analyzed by sanger sequencing (Macrogen, Europe) followed by inference of CRISPR editing software (ICE) (Synthego, CA, USA) analysis to validate every clonal edit (Figure S1B).

    Article Title: CRISPR-Cas9 globin editing can induce megabase-scale copy-neutral losses of heterozygosity in hematopoietic cells
    Article Snippet: The Inference of CRISPR Editing (ICE) software ( https://ice.synthego.com ) was used to determine the percentage of indels among polyclonal populations for all protocols allowing gRNA efficiencies by ICE (Synthego).

    Article Title: PEG-Delivered CRISPR-Cas9 Ribonucleoproteins System for Gene-Editing Screening of Maize Protoplasts
    Article Snippet: CRISPR-Cas9 DNA changes were calculated based on the insertions and deletions (In/Dels) around the cleavage site (3 bp upstream of the PAM sequence) using the Inference of CRISPR Editing Software—ICE software v.2 (Synthego Corporation, Palo Alto, CA, USA).

    Article Title: FOXD1 regulates cell division in clear cell renal cell carcinoma
    Article Snippet: Genomic DNA was extracted, PCR-amplified, sequenced using Sanger sequencing, and analyzed for editing efficiency using Synthego Inference of CRISPR Edits (ICE) software ( ice.synthego.com ).

    Article Title: CRISPR-Editing of Sweet Basil ( Ocimum basilicum L.) Homoserine Kinase Gene for Improved Downy Mildew Disease Resistance
    Article Snippet: The DNA sequencing chromatogram data for each T 0 and T 1 sample was analyzed using the Inference of CRISPR Editing (ICE) software tool (Synthego Performance Analysis, ICE Analysis.

    Article Title: The Solanum tuberosum GBSSI gene: a target for assessing gene and base editing in tetraploid potato.
    Article Snippet: Chromatograms from Sanger sequencing were analysed using the Inference of CRISPR Editing (ICE) software (Synthego, USA), an open-source and free-to-use Webbased tool (https ://ice.synth ego.com) (Hsiau et al. 2018).

    Generated:

    Article Title: Gene-edited basil plants resistant to downy mildew
    Article Snippet: The DNA sequencing chromatogram data for each TO and T1 sample was analyzed using the Inference of CRISPR Editing (ICE) software tool (Synthego Performance Analysis, ICE Analysis.

    Article Title: Oct4 is a gatekeeper of epithelial identity by regulating cytoskeletal organization in skin keratinocytes.
    Article Snippet: Genomic DNA from each OCT4 knockout clone or WT was extracted, amplified as above, and analyzed by sanger sequencing (Macrogen, Europe) followed by inference of CRISPR editing software (ICE) (Synthego, CA, USA) analysis to validate every clonal edit (Figure S1B).

    Article Title: CRISPR-Cas9 globin editing can induce megabase-scale copy-neutral losses of heterozygosity in hematopoietic cells
    Article Snippet: The Inference of CRISPR Editing (ICE) software ( https://ice.synthego.com ) was used to determine the percentage of indels among polyclonal populations for all protocols allowing gRNA efficiencies by ICE (Synthego).

    Article Title: PEG-Delivered CRISPR-Cas9 Ribonucleoproteins System for Gene-Editing Screening of Maize Protoplasts
    Article Snippet: CRISPR-Cas9 DNA changes were calculated based on the insertions and deletions (In/Dels) around the cleavage site (3 bp upstream of the PAM sequence) using the Inference of CRISPR Editing Software—ICE software v.2 (Synthego Corporation, Palo Alto, CA, USA).

    Article Title: FOXD1 regulates cell division in clear cell renal cell carcinoma
    Article Snippet: Genomic DNA was extracted, PCR-amplified, sequenced using Sanger sequencing, and analyzed for editing efficiency using Synthego Inference of CRISPR Edits (ICE) software ( ice.synthego.com ).

    Article Title: CRISPR-Editing of Sweet Basil ( Ocimum basilicum L.) Homoserine Kinase Gene for Improved Downy Mildew Disease Resistance
    Article Snippet: The DNA sequencing chromatogram data for each T 0 and T 1 sample was analyzed using the Inference of CRISPR Editing (ICE) software tool (Synthego Performance Analysis, ICE Analysis.

    Article Title: The Solanum tuberosum GBSSI gene: a target for assessing gene and base editing in tetraploid potato.
    Article Snippet: Chromatograms from Sanger sequencing were analysed using the Inference of CRISPR Editing (ICE) software (Synthego, USA), an open-source and free-to-use Webbased tool (https ://ice.synth ego.com) (Hsiau et al. 2018).

    Produced:

    Article Title: Gene-edited basil plants resistant to downy mildew
    Article Snippet: The DNA sequencing chromatogram data for each TO and T1 sample was analyzed using the Inference of CRISPR Editing (ICE) software tool (Synthego Performance Analysis, ICE Analysis.

    Article Title: Oct4 is a gatekeeper of epithelial identity by regulating cytoskeletal organization in skin keratinocytes.
    Article Snippet: Genomic DNA from each OCT4 knockout clone or WT was extracted, amplified as above, and analyzed by sanger sequencing (Macrogen, Europe) followed by inference of CRISPR editing software (ICE) (Synthego, CA, USA) analysis to validate every clonal edit (Figure S1B).

    Article Title: CRISPR-Cas9 globin editing can induce megabase-scale copy-neutral losses of heterozygosity in hematopoietic cells
    Article Snippet: The Inference of CRISPR Editing (ICE) software ( https://ice.synthego.com ) was used to determine the percentage of indels among polyclonal populations for all protocols allowing gRNA efficiencies by ICE (Synthego).

    Article Title: PEG-Delivered CRISPR-Cas9 Ribonucleoproteins System for Gene-Editing Screening of Maize Protoplasts
    Article Snippet: CRISPR-Cas9 DNA changes were calculated based on the insertions and deletions (In/Dels) around the cleavage site (3 bp upstream of the PAM sequence) using the Inference of CRISPR Editing Software—ICE software v.2 (Synthego Corporation, Palo Alto, CA, USA).

    Article Title: FOXD1 regulates cell division in clear cell renal cell carcinoma
    Article Snippet: Genomic DNA was extracted, PCR-amplified, sequenced using Sanger sequencing, and analyzed for editing efficiency using Synthego Inference of CRISPR Edits (ICE) software ( ice.synthego.com ).

    Article Title: CRISPR-Editing of Sweet Basil ( Ocimum basilicum L.) Homoserine Kinase Gene for Improved Downy Mildew Disease Resistance
    Article Snippet: The DNA sequencing chromatogram data for each T 0 and T 1 sample was analyzed using the Inference of CRISPR Editing (ICE) software tool (Synthego Performance Analysis, ICE Analysis.

    Article Title: The Solanum tuberosum GBSSI gene: a target for assessing gene and base editing in tetraploid potato.
    Article Snippet: Chromatograms from Sanger sequencing were analysed using the Inference of CRISPR Editing (ICE) software (Synthego, USA), an open-source and free-to-use Webbased tool (https ://ice.synth ego.com) (Hsiau et al. 2018).

    Incubation:

    Article Title: Gene-edited basil plants resistant to downy mildew
    Article Snippet: The DNA sequencing chromatogram data for each TO and T1 sample was analyzed using the Inference of CRISPR Editing (ICE) software tool (Synthego Performance Analysis, ICE Analysis.

    Article Title: Oct4 is a gatekeeper of epithelial identity by regulating cytoskeletal organization in skin keratinocytes.
    Article Snippet: Genomic DNA from each OCT4 knockout clone or WT was extracted, amplified as above, and analyzed by sanger sequencing (Macrogen, Europe) followed by inference of CRISPR editing software (ICE) (Synthego, CA, USA) analysis to validate every clonal edit (Figure S1B).

    Article Title: CRISPR-Cas9 globin editing can induce megabase-scale copy-neutral losses of heterozygosity in hematopoietic cells
    Article Snippet: The Inference of CRISPR Editing (ICE) software ( https://ice.synthego.com ) was used to determine the percentage of indels among polyclonal populations for all protocols allowing gRNA efficiencies by ICE (Synthego).

    Article Title: PEG-Delivered CRISPR-Cas9 Ribonucleoproteins System for Gene-Editing Screening of Maize Protoplasts
    Article Snippet: CRISPR-Cas9 DNA changes were calculated based on the insertions and deletions (In/Dels) around the cleavage site (3 bp upstream of the PAM sequence) using the Inference of CRISPR Editing Software—ICE software v.2 (Synthego Corporation, Palo Alto, CA, USA).

    Article Title: FOXD1 regulates cell division in clear cell renal cell carcinoma
    Article Snippet: Genomic DNA was extracted, PCR-amplified, sequenced using Sanger sequencing, and analyzed for editing efficiency using Synthego Inference of CRISPR Edits (ICE) software ( ice.synthego.com ).

    Article Title: CRISPR-Editing of Sweet Basil ( Ocimum basilicum L.) Homoserine Kinase Gene for Improved Downy Mildew Disease Resistance
    Article Snippet: The DNA sequencing chromatogram data for each T 0 and T 1 sample was analyzed using the Inference of CRISPR Editing (ICE) software tool (Synthego Performance Analysis, ICE Analysis.

    Article Title: The Solanum tuberosum GBSSI gene: a target for assessing gene and base editing in tetraploid potato.
    Article Snippet: Chromatograms from Sanger sequencing were analysed using the Inference of CRISPR Editing (ICE) software (Synthego, USA), an open-source and free-to-use Webbased tool (https ://ice.synth ego.com) (Hsiau et al. 2018).



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    Image Search Results


    Enhanced autophagic flux is an on-target effect of Selinexor in glioblastoma cells. ( A ) DNA sequence read from GBM43 cells ( bottom ) and GBM43 cells in which a CRISPR knock-in approach ( top ) encoding C528S mutant (mut) XPO1. ( B ) The visualization ( top ) and lowest bind energy ( bottom ) of molecular docking between Selinexor (Slx) and XPO1 wildtype/mut protein. ( C ) Representative immunofluorescence analysis ( top ) and quantitation ( bottom ) of GBM43 and GBM43mut cells incubated for 24 h with 0 or 1000 nM Selinexor, then analyzed for RanBP1 co-localization with nuclear DAPI staining (10–15 cells/group). ( D ) ( Left ) cell viability (normalized to control cells) of GBM43 and GBM43mut cell lines following 72 h continuous incubation with varying concentrations of Selinexor, and ( right ) IC50 values of Selinexor for GBM43 and GBM43mut cell lines. ( E ) Representative photos of clonogenic assays performed using GBM43 and GBM43mut cells continuously exposed to 0, 100, 200, or 400 nM Selinexor for 14 days. ( F ) Representative Western blot analysis of LC3B-I, LC3B-II, and ACTIN levels in GBM43 and GBM43mut cells lines incubated with DMSO, Selinexor (750 nM, 72 h) or rapamycin (500 nM, 16 h), or with non-targeted siRNA or a pool of siRNA targeting XPO1 (48 h after a 24-h siRNA incubation). ( G ) Viable number of GBM43 and GBM43mut cells (normalized to control cells) monitored daily after incubation with non-targeted siRNA or a pool of siRNA targeting XPO1 as in (F). Except where noted, all quantitated values listed are the means of 3 experiments. CYS, Cysteine; SER, Serine. ***, P ≤ .001.

    Journal: Neuro-Oncology

    Article Title: Autophagy modulates glioblastoma cell sensitivity to Selinexor-mediated XPO1 inhibition

    doi: 10.1093/neuonc/noae280

    Figure Lengend Snippet: Enhanced autophagic flux is an on-target effect of Selinexor in glioblastoma cells. ( A ) DNA sequence read from GBM43 cells ( bottom ) and GBM43 cells in which a CRISPR knock-in approach ( top ) encoding C528S mutant (mut) XPO1. ( B ) The visualization ( top ) and lowest bind energy ( bottom ) of molecular docking between Selinexor (Slx) and XPO1 wildtype/mut protein. ( C ) Representative immunofluorescence analysis ( top ) and quantitation ( bottom ) of GBM43 and GBM43mut cells incubated for 24 h with 0 or 1000 nM Selinexor, then analyzed for RanBP1 co-localization with nuclear DAPI staining (10–15 cells/group). ( D ) ( Left ) cell viability (normalized to control cells) of GBM43 and GBM43mut cell lines following 72 h continuous incubation with varying concentrations of Selinexor, and ( right ) IC50 values of Selinexor for GBM43 and GBM43mut cell lines. ( E ) Representative photos of clonogenic assays performed using GBM43 and GBM43mut cells continuously exposed to 0, 100, 200, or 400 nM Selinexor for 14 days. ( F ) Representative Western blot analysis of LC3B-I, LC3B-II, and ACTIN levels in GBM43 and GBM43mut cells lines incubated with DMSO, Selinexor (750 nM, 72 h) or rapamycin (500 nM, 16 h), or with non-targeted siRNA or a pool of siRNA targeting XPO1 (48 h after a 24-h siRNA incubation). ( G ) Viable number of GBM43 and GBM43mut cells (normalized to control cells) monitored daily after incubation with non-targeted siRNA or a pool of siRNA targeting XPO1 as in (F). Except where noted, all quantitated values listed are the means of 3 experiments. CYS, Cysteine; SER, Serine. ***, P ≤ .001.

    Article Snippet: Complete replacement of wildtype (WT) XPO1 with C528S mutant (mut) XPO1 was confirmed using Synthego’s Inference of CRISPR Edits (ICE) software tool (Synthego Performance Analysis, ICE Analysis, v3.0).

    Techniques: Sequencing, CRISPR, Knock-In, Mutagenesis, Immunofluorescence, Quantitation Assay, Incubation, Staining, Control, Western Blot

    A The Inference of CRISPR Edits (ICE) software output of the analyses of the Sanger sequencing data on the SYCP3 gene part flanking the exon 1 of the ORF. B Invasion analysis in DU145 cells using 10% FBS as a chemoattractant. Top panels, representative images of cells (bars: 100 µm); bottom panels, histograms showing the mean value ± S.E.M. of the percentage of invasive cells (n = 3). C Migration analysis of DU145 cells using a wound healing assay (n=3). D-F Adhesion/spreading analysis of DU145 cells. Representative images of adhered DU145 cells (C) and histograms showing the mean value ± S.E.M. of the percentage of adhered cells (D) or areas covered (E) of SYCP3 depleted cells referred to NTC cells (n = 3). Scale bars: 100 μm. G Immuno-fluorescence microscopy images of phalloidin staining ( red ) adhered NTC and SYCP3 depleted DU145 cells. Cell nuclei were stained with DAPI ( blue ). Scale bars: 100 μm. H-I Histograms showing the cytoskeleton (G) or nuclei area value ± S.E.M. of adhered DU145 indicated cells (n=2). Statistic tests: Student t test ( B, C ), two-way ANOVA ( E, F ) and U-Mann Whitney test ( H, I) . *, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001.

    Journal: bioRxiv

    Article Title: CRISPR screening reveals SYCP3 as a key driver of metastasis in prostate cancer

    doi: 10.1101/2025.01.30.629925

    Figure Lengend Snippet: A The Inference of CRISPR Edits (ICE) software output of the analyses of the Sanger sequencing data on the SYCP3 gene part flanking the exon 1 of the ORF. B Invasion analysis in DU145 cells using 10% FBS as a chemoattractant. Top panels, representative images of cells (bars: 100 µm); bottom panels, histograms showing the mean value ± S.E.M. of the percentage of invasive cells (n = 3). C Migration analysis of DU145 cells using a wound healing assay (n=3). D-F Adhesion/spreading analysis of DU145 cells. Representative images of adhered DU145 cells (C) and histograms showing the mean value ± S.E.M. of the percentage of adhered cells (D) or areas covered (E) of SYCP3 depleted cells referred to NTC cells (n = 3). Scale bars: 100 μm. G Immuno-fluorescence microscopy images of phalloidin staining ( red ) adhered NTC and SYCP3 depleted DU145 cells. Cell nuclei were stained with DAPI ( blue ). Scale bars: 100 μm. H-I Histograms showing the cytoskeleton (G) or nuclei area value ± S.E.M. of adhered DU145 indicated cells (n=2). Statistic tests: Student t test ( B, C ), two-way ANOVA ( E, F ) and U-Mann Whitney test ( H, I) . *, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001.

    Article Snippet: DNA sequencing chromatograms were analyzed using the Inference of CRISPR Edits (ICE) free software tool (synthego.com/products/bioinformatics/crispranalysis).

    Techniques: CRISPR, Software, Sequencing, Migration, Wound Healing Assay, Fluorescence, Microscopy, Staining, MANN-WHITNEY